Islet isolation for allotransplantation: variables associated with successful islet yield and graft function
نویسندگان
چکیده
منابع مشابه
Introducing a New Experimental Islet Transplantation Model using Biomimetic Hydrogel and a Simple High Yield Islet Isolation Technique
Background: Islet transplantation could be an ideal alternative treatment to insulin therapy for type 1 diabetes Mellitus (T1DM). This clinical and experimental field requires a model that covers problems such as requiring a large number of functional and viable islets, the optimal transplantation site, and the prevention of islet dispersion. Hence, the methods of choice for isolation of functi...
متن کاملHuman islet isolation and allotransplantation in 22 consecutive cases.
This report provides our initial experience in islet isolation and intrahepatic allotransplantation in 21 patients. In group 1, 10 patients underwent combined liver-islet allotransplantation following upper-abdominal exenteration for cancer. In group 2, 4 patients received a combined liver-islet allograft for cirrhosis and diabetes. One patients had plasma C-peptide greater than 3 pM and was th...
متن کاملOptimizing Porcine Islet Isolation to Markedly Reduce Enzyme Consumption Without Sacrificing Islet Yield or Function
BACKGROUND Human allogeneic islet transplantation for treatment of type 1 diabetes provides numerous clinical benefits, such as fewer episodes of hypoglycemic unawareness and tighter control of blood glucose levels. Availability of human pancreas for clinical and research use, however, is severely limited. Porcine pancreas offers an abundant source of tissue for optimization of islet isolation ...
متن کاملSuccessful human islet isolation utilizing recombinant collagenase.
The enzymatic dissociation of acinar tissue by collagenase is a substantial step in the isolation of pancreatic islets. Although essential collagenase components have been purified, the variability in the activity of different batches limits long-term reproducibility of isolation success. The utilization of purified recombinant proteases would solve this problem. In the present study, pancrease...
متن کاملDetection of pancreatic islet tissue following islet allotransplantation in man.
Qualitative and quantitative standards have been recently proposed for islet isolation asaeument in humans and large animals ( 1). The ultimate test of viability is functional activity after transplantation into a diabetic recipient. Morphologic identification of insulin-containing transplanted islets could provide important correlative data and eventually confmn the functional results. Recentl...
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ژورنال
عنوان ژورنال: Diabetologia
سال: 2005
ISSN: 0012-186X,1432-0428
DOI: 10.1007/s00125-005-1725-3